Journal:

Article Title: Mycobacterium tuberculosis Catalase and Peroxidase Activities and Resistance to Oxidative Killing in Human Monocytes In Vitro

doi:

Figure Lengend Snippet: M. tuberculosis strains

Article Snippet: The culture medium was lipopolysaccharide free and without reactivity in the Limulus amoebocyte lysis assay (Whittaker Bioproducts, Walkersville, Md.). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Mycobacterial strain Protein expression a KatG AhpC Reference laboratory strain H37Rv + +/− Clinical isolates CDC 1551 + ND b ATCC 35825 (Inh r ) − + Recombinant strains H37Rv Inh r − +/− H37Rv(pMH59) +++ +/− H37Rv(pMH91) + +++ Open in a separate window a +/−, detectable by Western blotting but not by Coomassie blue staining of the gel; +, detectable by Coomassie blue staining; +++, overexpressed as Coomassie blue staining. b ND, not determined.

Techniques: Expressing, Recombinant

Journal:

Article Title: Mycobacterium tuberculosis Catalase and Peroxidase Activities and Resistance to Oxidative Killing in Human Monocytes In Vitro

doi:

Figure Lengend Snippet: Growth rate of M. tuberculosis strains (in vitro)

Article Snippet: The culture medium was lipopolysaccharide free and without reactivity in the Limulus amoebocyte lysis assay (Whittaker Bioproducts, Walkersville, Md.). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Mycobacterial strain Protein expression a KatG AhpC Reference laboratory strain H37Rv + +/− Clinical isolates CDC 1551 + ND b ATCC 35825 (Inh r ) − + Recombinant strains H37Rv Inh r − +/− H37Rv(pMH59) +++ +/− H37Rv(pMH91) + +++ Open in a separate window a +/−, detectable by Western blotting but not by Coomassie blue staining of the gel; +, detectable by Coomassie blue staining; +++, overexpressed as Coomassie blue staining. b ND, not determined.

Techniques: Recombinant

Journal:

Article Title: Mycobacterium tuberculosis Catalase and Peroxidase Activities and Resistance to Oxidative Killing in Human Monocytes In Vitro

doi:

Figure Lengend Snippet: Effect of exogenous H2O2 on mycobacterial viability in cell-free Middlebrook 7H9 medium. H2O2 (at the concentrations shown) was added to growing bacteria, and the number of CFU was determined as described in Materials and Methods. Results are expressed as percent survival relative to baseline ± SEM and are from one representative experiment with six replicate cultures. The numbers of CFU used for the experiment (and expressed as 100% in the figure) were 4.7 × 105 for H37Rv, 3.2 × 105 for H37Rv Inhr, and 2.8 × 105 for H37Rv(pMH59).

Article Snippet: The culture medium was lipopolysaccharide free and without reactivity in the Limulus amoebocyte lysis assay (Whittaker Bioproducts, Walkersville, Md.). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Mycobacterial strain Protein expression a KatG AhpC Reference laboratory strain H37Rv + +/− Clinical isolates CDC 1551 + ND b ATCC 35825 (Inh r ) − + Recombinant strains H37Rv Inh r − +/− H37Rv(pMH59) +++ +/− H37Rv(pMH91) + +++ Open in a separate window a +/−, detectable by Western blotting but not by Coomassie blue staining of the gel; +, detectable by Coomassie blue staining; +++, overexpressed as Coomassie blue staining. b ND, not determined.

Techniques:

Journal:

Article Title: Mycobacterium tuberculosis Catalase and Peroxidase Activities and Resistance to Oxidative Killing in Human Monocytes In Vitro

doi:

Figure Lengend Snippet: PMA-induced killing of intracellular mycobacteria. Monocytes were infected with H37Rv Inhr (circles) or H37Rv(pMH59) (squares). Intracellular bacilli (solid lines) and cell free bacilli (broken lines) were treated with PMA at a final concentration of 100 ng/ml. The open symbols indicate controls not treated with PMA. Results are expressed as mean CFU ± standard deviation of three experiments, each done in triplicate. Data were analyzed with a paired t test to compare control and PMA-treated cells. Significantly different results for controls versus corresponding PMA-treated cells are indicated by ∗ (P = 0.02) and ∗∗ (P = 0.04).

Article Snippet: The culture medium was lipopolysaccharide free and without reactivity in the Limulus amoebocyte lysis assay (Whittaker Bioproducts, Walkersville, Md.). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Mycobacterial strain Protein expression a KatG AhpC Reference laboratory strain H37Rv + +/− Clinical isolates CDC 1551 + ND b ATCC 35825 (Inh r ) − + Recombinant strains H37Rv Inh r − +/− H37Rv(pMH59) +++ +/− H37Rv(pMH91) + +++ Open in a separate window a +/−, detectable by Western blotting but not by Coomassie blue staining of the gel; +, detectable by Coomassie blue staining; +++, overexpressed as Coomassie blue staining. b ND, not determined.

Techniques: Infection, Concentration Assay, Standard Deviation